高考生物:现代生物技术

A high-school biology practice set on modern biotechnology: gel electrophoresis of DNA, genetic engineering with restriction enzymes and ligases, plant cell fusion, animal cell culture, embryo transfer, nuclear transfer cloning, and marker genes.

  • Explain what factors affect DNA fragment migration in gel electrophoresis
  • Describe risks of using a single restriction enzyme in vector construction
  • Compare plant cell fusion and animal cell fusion techniques
  • Explain the role of the oocyte cytoplasm in somatic cell nuclear transfer

高考生物:现代生物技术 — PCR、凝胶电泳、基因工程、细胞工程、胚胎工程与克隆基础。

示例问题

在进行琼脂糖凝胶电泳分析DNA片段时,下列哪些因素会影响DNA分子的迁移速率?

查看答案

DNA分子的相对分子质量, 凝胶的浓度, DNA分子的构象

DNA在电场中受力移动,迁移速率受分子大小(质量)、凝胶孔径(由浓度决定)以及分子形状(构象,如超螺旋、开环、线性)影响。缓冲液颜色不影响电泳迁移。

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